Microgels and apparatus for PAGE of nucleic acids in one or two dimensions

Author:

Thormar Hans G12,Gunnarsson Gudmundur H12,Gudmundsson Bjarki132,Leosson Kristjan4,Estibeiro Peter2,Jonsson Jon J13

Affiliation:

1. Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Iceland, Reykjavik, Iceland

2. Lifeind ehf, Reykjavik, Iceland

3. Department of Genetics & Molecular Medicine, Landspitali – National University Hospital, Reykjavík, Iceland

4. Department of Materials Science, Biotechnology & Energy, Innovation Center, Reykjavik, Iceland

Abstract

We describe a system for horizontal 1D or 2D PAGE comprising an apparatus and microgels. There is no buffer outside the gel, making handling and sample loading easy. Specially designed electrodes on all four sides allow 2D electrophoresis without gel rotation. Electrophoresis is completed within 20 min and sensitivity is in the subnanogram range. The system is temperature controlled for speed, denaturation of nucleic acid molecules and maintaining molecules single-stranded. The system allows characterization of structure, conformation and damage in complex nucleic acid preparations. Besides quick 1D PAGE, 2D applications include characterization of efficiency of complex molecular procedures, checking quality of biosamples and detecting DNA damage in cells and body fluids. The system should also run protein gels.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

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