Cilia proteins CFAP36 and sentan in induced sputum as possible new markers of epithelial damage in obstructive lung diseases: A preliminary study

Abstract

Background: Asthma and chronic obstructive pulmonary disease (COPD) are the most common chronic respiratory diseases characterized by inflammation in the lower airways and epithelium remodeling. Dysfunction of cilium is related to severe asthma and COPD, the role of cilium proteins in obstructive lung diseases is not known. The aim of the study was to evaluate the concentration of cilia associated proteins: sentan and CFAP36 in induced sputum (IS) of asthma and COPD patients. Materials/Methods: The study involved 15 patients with asthma, 12 patients with COPD and 17 control subjects (9 non-smoking, 8 smoking) who underwent lung function tests and sputum induction. Sentan, CFAP36, IL-6, IL-8, concentrations were measured in induced sputum supernatants by ELISA. Results: The level of CFAP36 in induced sputum was elevated in asthma patients and subjects with atopy. Cilium protein levels in sputum were not related to spirometric tests results. Both CFAP36 and sentan concentrations were positively correlated with age. The level of sentan was associated with airway neutrophilic inflammation and active smoking status. CFAP36 concentration was negatively related to cell viability, whereas sentan level was positively related, but only in COPD patients. Conclusions: The results of our study revealed CFAP36 and sentan as possible new markers of epithelial damage of different origin in obstructive lung diseases.