Aqueous Two-Phase Separation (ATPS) Methods for Oleic acid extraction from Neem leaves

Abstract

Abstract The aqueous two-phase separation system (ATPS) signifies an environmentally responsible approach for the extraction of bioactive compounds from a plants basis, as it is a liquid-liquid fractionation technique centred on the inconsistency of two aqueous solutions. In this investigation, various experimental parameters are optimized as the speed of agitation (200, 300 400 and 500 rpm) and solvent ratio (1:1, 2:3 and 3:2) with 20 % (w/w) of Ammonium Sulphate (AMS) salt composition and 30 % (w/w) of Polyethelyene Glycol (PEG). The obtained extract contains alkaloids, flavonoids, tannins, glycosides, acids and total phenolic compounds (TPC). The extracted Oleic acid by the ATPS method was measured with gallic acid equivalent (GAE) of TPC extracted from neem leaves powder. The determined concentration of oleic acid in the practice of TPC is 8.033 mg of GAE/g from the optimized experimental parameter. The optimized results can be cast off for a commercial process on an industrialized scale. Also, the mathematical modelling investigation was done to intent the critical impeller speed (Njs) with the Zwittering model. The identified model calculates the essential speed of agitation (rpm) for maximum extraction yield.