In vitro study of DNA-adduct formation (8-OHdG) due to exposure of benzo[a]pyrene and Co(II) through fenton-like reaction

Abstract

Abstract Severely increasing environmental pollution with an unhealthy pattern of life in the community can increase the source of exposure of carcinogenic substances that can cause cancer. Benzo[a]pyrene and Cobalt (II) are some examples of carcinogenic substances commonly found in the environment. Both materials can simultaneously enter the body through food or polluted air and damage the DNA. In this study, we studied the effect of B[a]P and Co(II) exposure in-vitro on 2’-deoxyguanosine. An increase in the amount of the 8-hydroxy-2’-deoxyguanosin compound is used as an indicator of the occurrence of DNA damage and a cancer risk biomarker. It was found that the synergistic effect on the combination of B[a]P and Co (II) through Fenton-like reaction is influenced by the concentration of H2O2. The amount of 8-OHdG that formed with the combination of B[a]P and Co (II) is 126.23 ppb. It does not vary much with the amount that resulted from B[a]P exposure alone at 136.53 ppb. Exposure of Co(II) through Fenton-like reaction alone gives the highest yield of 8-OHdG formation at 282.67 ppb. Followed by B[a]P and Co(II) at 258.98 ppb, B[a]P and H2O2 at 153.16 ppb.