Application of sensor structures based on a photoelectric transducer to determine the activity of aspartate and alanine aminotransferases in blood plasma

Abstract

Abstract The successful application of a recombination sensor for the real-time detection of transaminasethe detection of transaminase activities (ALT/AST) in the blood plasma of rats has been demonstrated. The parameter directly measured in real time is the photocurrent through the structure with buried silicon barrier when light with high absorption coefficient is used. Detection is realized as a result of specific chemical reactions catalyzed by ALT and AST enzymes (α-ketoglutarate + aspartate and α-ketoglutarate + alanine). The change of the effective charge of the reagents allows recording the activity of enzymes from photocurrent measurements. The main factor in this approach is the influence on the parameters of the recombination centers at the interface. The physical mechanism of the sensor structure can be explained within the framework of the Stevenson theory, taking into account the changes in the pre surface band bending, the capture cross sections and the energy position of the recombination levels during adsorption. The paper also offers theoretical analyze allowing optimization of analytical signals of recombination sensor. A promising approach to develop a simple and sensitive method for real time detection of transaminases activity has been discussed in detail.