Antibacterial activity of ethanol extract, n-hexan, ethyl acetate and butanol fraction of Momordica charantia L. seed against Staphylococcus epidermidis

Abstract

Abstract Acne is a skin disease caused by excessive production of oil, causing clogged pilosebaceous follicular ducts and inflammation. One of the bacteria triggered acne is Staphylococcus epidermidis. Acne treatment usually uses antibiotics that inhibit inflammation and bacterial growth. To reduce the effects of using chemical drugs, research is needed to find natural treatment solutions such as using the bitter melon plant. The part that is often used from bitter melon is flesh of the fruit, while the seeds are only used as waste, so researchers are interested in examining the antibacterial activity of bitter melon seeds. Tests were carried out on ethanol extract, fraction of n-hexane, ethyl acetate and butanol in bitter melon (Momordica charantia L.) seeds against Staphylococcus epidermidis by determining the minimum bakterisid concentration (MBC) using the liquid dilution method of a concentration of 80%; 40%; 20%; 10%; 5%; 2.5%; 1.25% and 0.625%. The value of minimum bakterisid concentration (MBC) is indicated by the absence of colony growth on agar media. The minimum bakterisid concentration (MBC) in this study was only obtained in the ethyl acetate fraction, at a concentration of 40%, while in extracts and other fractions the MBC value was not obtained.