Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and Antioxidant Activity of Sea-Cucumber (Holothurian atra and Holothurian edulis) From Selayar Island

Abstract

Abstract The present investigation was carried out to characterize the bioactive compounds present in extract of Holothurian atra and Holothurian edulis using Gas Chromatography-Mass Spectrum (GC-MS) and antioxidan activity. The results of the GC-MS analysis provide different peaks determining the presence of 4 phytochemical compounds with different therapeutic activities. The measurement of antioxidant activity carried out using 1,1difenil-2-pikrihidazil (DPPH) method. The major, 9-Octadecenoic acid (Z)-, methyl ester (47.29), hexadecanoic acid, methyl ester (28.41), octadecanoic acid, methyl ester (4.47), and [1,1′-bibicyclo[2.2.2]Octane]-4-carboxylic acid (92.11) and minor compounds were also present. Antioxidant activity of the ethanolic extract of Holothurian edulis have low antioxidant activity with the value IC50 41.6828 mg/mL and ethanolic extracts of Holothurian atra have strong antioxidant activity with value IC50 33.514 mg/mL. From the results, it could be concluded that Holothurian atra and Holothurian edulis contains various bioactive compounds have various biological activities and antioxidant activity.