Silver nanoparticle-based biosensor as a rapid test for the detection 3-monochloropropane-1,2-diol (3-MCPD) in cooking oil

Abstract

Abstract One of the toxic compounds that are carcinogenic (cancer causing) in food is chloropropanol compound. These compounds are currently of great concern considering the increasing number of cancer and kidney failure sufferers in the world apart from degenerative diseases. Derivatives of these compounds are widely found in food ingredients, such as vegetable oils, baby food, smoked fish, infant formula, processed soybeans, margarine, cereals, soy sauce, and others. ). There are several types of chloropropanol compounds with one or two chlorine atoms in glycerol at position 1 or 2 such as 3-monochloropropane-1,2-diol (3-MCPD), 2-monochloropropane-1,3-diol (2-MCPD). Therefore, early prevention is needed for further treatment through the detection of 3-MCPD in cooking oil. The development that will be carried out is a silver nanoparticle-based biosensor combined with colorimetric. The method used to obtain a suitable biosensor is by synthesizing silver nanoparticles (AgNPs) coated with cystein. The content of 3-MCPD in the new oil is 2,828 µg/Kg. Meanwhile, used cooking oil has a higher content of 3-MCPD, that is as much 19,042 µg/Kg. This value exceeds the maximum limit set by the European Commission (EC), which is 1,250 µg/Kg for oils/fats sourced from coconut, corn, sunflower, palm kernel oil and olive oil.