Author:
Pienasthika M,Brahmanti A A,Purwatiningrum I,Wardani A K
Abstract
Abstract
The best fermentation ability of yeast-forming flock species is highly desirable in the bioethanol production process. Saccharomyces cerevisiae NCYC 1195 is known to have the ability to form flock which is influenced by the concentration of calcium chloride (CaCl2) and pH. This is because calcium ions can form bonds with mannose on the surface of the cell wall of S. cerevisiae, while the degree of acidity will affect the charges of specific protein on the cell wall. The ability of flocculation will precipitate S. cerevisiae cells so that they will not be mixed with fermentation medium. Thus, the separation process of cell and fermentation media can be done quickly and efficiently, without expensive centrifugation step. The aim of this study was to determine the effect of CaCl2 concentration and pH that used to determine the ability and stability of the flock of S. cerevisiae. Three concentration of CaCl2 (10-5, 10-7, 10-9 M) were used as well as three variations of pH (4,5,6). Culture was incubated at 30°C with 100 rpm agitation and analysed for flocculation ability every 4 hours during 24 hours. The stability of the flock was analysed every 24 hours during 30 days. The highest flock formation ability (73.15%) was obtained with the concentration of CaCl2 concentration 10-9 M and pH 5.
Reference28 articles.
1. Separation of yeast by addition of flocculent cells of Sacharomycess cerevisiae;Mortier;World J. Microbiol. Biotechnol.,2007
2. Repeated-batch fermentation using flocculant hybrid, Saccharomyces cerevisiae CHFY0321 for efficient production of bioethanol;Choi;Biotechnol. Prod. Process Eng.,2009
3. Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae;Ramos;Braz. J. Microbiol.,2013
4. Bioethanol from second generation feedstock (lignoselulose biomass);Idi;Interdis. J. Contem. Res. Bus.,2011
5. Genetic modification of industrial yeast strain to obtain controllable newflo flocculation property and lower diacetyl production;Wang;Biotechnol. Lett.,2008
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