The effect of degradation time in the simulated gastric fluid for bioactive compounds from bekasam

Abstract

Abstract Bekasam contains bioactive compounds (lovastatin and peptide) that has function as anticholesterol. This research was aimed to determine the effect of pH and degradation time to lovastatin, peptides, and dissolved protein in bekasam seluang extract (Rasbora sp.). This study used a laboratory experimental method with 4 levels of treatment (degradation in simulation gastric fluid pH 2, pH 3 pH 4 and control without simulated gastric fluid). The degradation results were observed every the 2nd hours, the 4th hours, and the 6th hours. Each treatment level repeated 3 times. The stages of the study consisted of making bekasam, extracting of bekasam, calculating the yield of bekasam extract and testing pure bekasam extract in simulated gastric fluid. The analysis was carried out using a descriptive method, which is to describe each parameter obtained. The parameters observed are analysis of lovastatin level, dissolved protein levels, and peptide levels. The results showed that the levels of lovastatin, dissolved proteins and peptides were still detectable after have degraded in simulation gastric fluid an acidity level of pH 2, pH 3 and pH 4 for 2nd to 6th hours.