Solvent selection for extraction of bioactive compound from bekasam with antioxidant and anticholesterol activities

Abstract

Abstract Heart disease and hypertension are two of the main causes of human mortality worldwide. Both can be caused by the oxidation of cholesterol, causing plaque and narrow blood vessels. The purpose of this research was to determine the solvent for the extraction of antioxidant and anticholesterol bioactive compounds from bekasam extracts. Bekasam was extracted by polar solvents, i.e., aquabides, methyl alcohol (methanol), and ethyl alcohol (ethanol). The parameters observed in this research were the yield of crude extract, antioxidant assay, lovastatin assay, and peptide profile analysis (SDS-PAGE). The result of this study indicated that the yields of crude extract of bekasam using aquabides, methanol, and ethanol were 17.00%, 9.00%, and 2.83%, respectively. The antioxidant activities of bekasam extracts produced using methanol, ethanol, and aquabidest were 69.09%, 64.09%, and 53.43%, respectively. Lovastatin contents of bekasam extracts produced using ethanol, aquabides, and methanol were 442.53 ppm, 168.67 ppm, and 158.19 ppm, respectively. Peptides were detected on bekasam extract by aquabidest in the range of 5.60-29.26 kD but undetected when extracted using the other solvents.