Effect of solvent ratio on omega-3 extraction of catfish (Clarias sp.) and pangasius catfish (Pangasius sp.) using low-temperature crystallization method

Abstract

Abstract Freshwater fish are not major of omega-3 fatty acids (FA). More ways needed to help improving omega-3 substance by yielding concentrate using a low-temperature crystallization technique. The intention of yielding omega-3 concentrate is to obliterate elements except omega-3 FA. This study aimed to determine the acetone solvent-oil ratio for acquiring the finest characteristic of omega-3 catfish (Clarias sp.) and pangasius catfish (Pangasius sp.) by analyzing iodine number (IV), oxidation parameters, and FA profiles. Omega-3 concentrate was made using a low-temperature crystallization technique with solvent-to-oil ratios of 3:1(v/v), 4:1(v/v), 5:1(v/v), 6:1(v/v), and 7:1(v/v). The chosen treatment was 5:1 (v/v) solvent-to-oil ratio, resulting in an increase in omega-3 content in catfish 3.65% and pangasius catfish 2.30%. The concentration of catfish and pangasius catfish increased by 1.69 and 1.28 times, respectively. The viscera of catfish and pangasius catfish belly obtained from by-products of fish fillet industries and markets have the potential to be a basic material in the formation of omega-3-rich FO.