Endo-xylanase Enzyme Production using Agroindustrial Biomass as Feedstock by Kitasatospora sp.

Abstract

Abstract Endo-β-1, 4-xylanases is an enzyme that depolymerize xylan, a major component of lignocelluloses. Lignoselulose is a great source of cheap carbohydrate and thus has been used over the past decade as a raw material for the production of high value products, such as enzymes. However, lignocelluloses are a highly recalcitrant material that is extremely difficult to depolymerize. By using proper pretreatment, lignocelluloses from agroindustrial biomass can replace conventional carbon sources in media preparation for enzyme production. In this study, we used three kinds of agroindustrial biomass, such as sorghum variety of Buleleng, sorghum variety of JP, rice straw LIPI GO1 and these biomasses were pretreated with acid to remove a portion of lignin for use as a carbon source to endoxylanase production. The production of xylanase Kitasatospora sp. under submerged fermentation was investigated with different carbon sources using agroindustrial biomass. Optimization steps included studies carbon source concentration and pH medium fermentation. The optimized condition of enzyme production was obtained using the sorghum variety Buleleng biomass at 2% concentration and pH medium is 9.0 with activity 3. 04 U/mL.