The Effect of Grape Seed Extract on Lipid Profile in Diabetic Mice

Abstract

Abstract The aim of this work is to evaluate the role of Grape Seed extracts (GSE) from three grape cultivars (Ahmer, Halawani, and Kamali) grown in Iraq as well as pomace on lipid profile in diabetic mice. Fifty adult female mice were divided into five groups. Negative control (untreatment), positive control (treated) diabetic, and diabetic mice treated with GSE. After six weeks; serum and liver tissue homogenate parameters were evaluated. HPLC results showed that Ahmer (red grape seeds) has highest concentration of proanthocyanidins polymers catechine, procyanidin, and epicatechine (796, 170, and 244) μg/g, respectively For that it was chosen for mice dosage, while the lowest amounts were in pomace 489, 99, and 143 μg/g, respectively. Oral dose of grape seed natural extract (600 mg/kg/day) reduced the level of lipid profile: TC, TG, LDL, and VLDL, (106.83±2.13, 84.66±4.27,13.83±1.16 and 21.33±1.21) respectively, except HDL which respectively was increased to 76.33±1.03. GSE were normalized the elevated levels of kidneys and liver functions. Similarly, Liver histological analysis shows that GSE groups exhibited morphology near to that of the control group.