Isolation and characterization of chitosan from fish scale waste

Abstract

Abstract This research aims to study the isolated conditions that affect the characteristics of chitosan from fish scale waste which was received from fermented fish factory. The chitosan was isolated by three steps which are deproteinization, demineralization, and deacetylation. In deacetylation steps, the chitosan from fish scale was produced under conditions of 35, 45, and 55°C with 2 M NaOH for 5 hr and a ratio between chitin and NaOH equal to 1:4, 1:6, and 1:8 (w/v). The experimental results showed that the ratio between fish scale and NaOH and deacetylation temperature influenced on yield, L*, b*, and deacetylation degree of the chitosan. The yield values of the chitosan were in range of 24.94-49.84%. The chitosan had deacetylation degree was in the range of 19.17-21.64%. The viscosity of the chitosan solution was approximately 15.66 cP. The outer surface of the chitosan is rough. Moreover, the Staphylococcus aureus inhibit activity of the chitosan was not presented.