Genetic marker exploration of fertility genes IGF 1 and IGF 2 at Ongole Cross breed cattle with naturally twin birth

Abstract

Abstract The phenomenon of beef cattle with twin births naturally is still unknown. The aim of this research is to explore genetic marka of IGF1 and IGF 2 genes as regulators of fertility in beef cattle with twin births naturally. Ongole Cross (OC) breed catte, each 10 cows with single or twin births naturally in Beef Cattle Research Station, take blood to make plasma samples according to the Boyan method, than make DNA isolation samples according to the QIAamp method, to be PCR analysis according to the Perkin-Elmer method and to be sequencing according to the Phenol-Chloroform method, in the Tropical Disease Center (TDC) Hospital, Airlangga University. The primers used are IGF 1 forward 5’-CCTCTGCGGGGCTGAGTTGGT-3’, reverse 5’-CGACTTGGCGGGCTTGAGA GGC-3’ and IGF 2 forward 5’-TCTGTGCGGCGGGGAGCTGGT-3’, reverse 5’-AGTCTCCAGCA GGGCCAGGTCG-3. Parameters observed: allele band, genotypic diversity and nucleotide acids composition of IGF genes. Allele band variation data is processed with Nt programe software to determine its genotype, nucleotide gene sequens data is processed using the GENETIC MAC version 8 program to determine gemone mutation construction of IGF, then presented descriptively. The results showed: the spread of IGF 1 allele band in cows with single birth at position 875–1200 bp and twin birth at position 875–1485 bp; spreading of IGF 2 allele band in cows with single birth at position 450-590 bp and twin birth at position 455–1110 bp; genotype diversity of IGF 1 genes at cows with single birth was homozygous AA with allele size 920–1200 bp and twin birth was heterozygote AB with allele size A 875-1150 bp and B allele 1250–1485 bp; genotype diversity of IGF 2 genes in cows with single birth was homozygous AA with allele size 460–560 bp and twin birth was heterozygote AB with allele size A 450-590 bp and B allele 940–1110 bp; at certain positions and nucleotide acids arrangement in both IGF 1 and IGF 2 genes of cows with single birth, was different with twin birth. It was concluded, the genetic marka of IGF 1 and IGF 2 genes of OC breed cattle, were different among cows with single birth and cows with twin birth.