The effects of apple juice (Malus sylverstris-Mill) as a natural antioxidant on spermatozoa viability of Tor soro 24 hours postcryopreservation

Abstract

Abstract The population of Tor soro in Indonesia continues to decline due to overfishing. In T. soro farming, the gonad maturity between males and females tends to be asynchronous. Thus, it is necessary to have a stock of milt that can be used at any time for fertilization of the eggs by cryopreservation. Cryopreservation can cause cell damage due to oxidative stress and can be reduced by the addition of antioxidants in the cryopreservation medium. Apple (Malus sylverstris-Mill) can act as an additional antioxidant to increase the chance of spermatozoa survival post-cryopreservation. This study aims to study the influence of adding various concentrations of apple juice (0 %, 10 %, 20 %, and 30 %) and determine which is most optimal in maintaining the viability of T. soro spermatozoa 24 hours post-cryopreservation with a 1:9 milt-diluent ratio. The mean viability of T. soro spermatozoa post-cryopreservation in 0 %, 10 %, 20 %, and 30 % concentrations are 12.01 ± 2.24 %, 8.10 ± 4.53 %, 53 ± 5.14 %, and 6.23 ± 4 % respectively. The results of the one-way ANOVA test showed that the apple juice in various concentrations did not affect the viability of T. soro spermatozoa 24 hours post-cryopreservation (P>0.5).