Studying the polymorphism of cereal varieties using RAPD and ISSR markers

Abstract

Abstract The sustainable development of the crop industry critically depends on increasing the stability of cultivated species through accelerated selection, selection of crops and varieties that complement each other, their adaptive zoning and increasing the varietal diversity of agroecosystems. Therefore, the analysis of the genetic structure of the gene pool and the assessment of the degree of genetic kinship using molecular genetic methods is necessary for achieving the most effective use of genetic resources. This study identified 28 polymorphic RAPD (random amplified polymorphic deoxyribonucleic acid) and ISSR (inter-simple sequence repeats) loci in spring triticale, wheat and oat varieties. The genetic distances of the studied varieties and breeding lines were calculated and found to vary from 0 to 1 for different markers. The data presented in this study should be utilised in future breeding research as it presents important insights into the hybridization and crossing of the source material located at a large genetic distance from each other.