Influence of deficient nutrition on trace element status and antioxidant defense system

Abstract

Abstract The aim of research was to study the effect of unbalanced nutrition on rat’s liver and hair element composition and antioxidant defense system in the experiment. The study was conducted on male Wistar rats. The experimental group was on mineral deficient diet, the control group received a standard diet. The elemental composition of the liver and hair was determined by atomic emission and mass spectrometry with inductively coupled argon plasma. Blood biochemical parameters were determined spectrometrically using a Clima MC-15 A/O Unimed analyzer. The level of malondialdehyde and the activity of glutathione peroxidase in the liver of animals were determined by standard methods using ELISA kits. Results. A decrease in the content of copper, zinc, and selenium was found in liver tissue by 1.5, 1.14, and 3.2 times, respectively. Significant increase of lead and aluminum was established. A similar changes were observed in the elemental composition of hair. GPx activity decreased in 1.4 times and MDA level increased in the liver of animals of the experimental group. An increase in ALT, AST and total protein was noted on mineral-deficient diet.