Design and development of temporary immersion bioreactor system controlled by microcontroller

Abstract

Abstract This research aims to develop, design and construct a temporary immersion bioreactor (TIB) controlled by microcontroller. The designed TIB can control the time for plant feeding and the carbon dioxide (CO2) concentration via application. The TIB consists of 2 sets of the plant tissue culture containers. The TIB can define the feeding and the CO2 concentration control times up to 10 and 4 time periods per day, respectively. The system feeds the plants and controls the CO2 concentration during the defined time periods. The test results of the feeding time control illustrated that the TIB could set the feeding times conveniently and quickly. Moreover, the system could properly work following the set times. For the test results of the CO2 concentration control, it was found that the TIB could control the CO2 concentration in the containers during the set operating time. It took about 7, 28, 30, 36 and 21 minutes after the system started working to adjust the CO2 concentration in order to be at the set levels of <750, 1,500, 2,000, 2,500 and 3,000 ppm, respectively. The CO2 concentration control system could properly work with error less than 10%.