Association of HSP70 gene with milk yield and milk quality of Friesian Holstein in Indonesia

Abstract

Abstract The aim of this study was to identify the polymorphism of the ScrFI locus of HSP70 and the association of genetic variant of HSP70 with milk yield and milk quality of Holstein-Friesian (HF) cows in Indonesia. A total of 115 heads of HF lactating cows which come from different locations of lowland, medium, and highland. Genotyping of HSP70 genes was performed by nested PCR-RFLP method. The variant of HSP70 was polymorphism: 47 and 49 bp for homozygous wild type (CC) genotype and 47, 49 and 96 for heterozygous cytosine deletion mutant (C-) genotype. Dairy cows with CC genotypes were higher 4%FCM, protein, fat, milk total solid and lactose (P <0.05) compare to C-genotype. It could be concluded that dairy cows with CC genotypes produce higher 4% FCM and better milk quality than dairy cows with C-.