Author:
Nadzirah Sharipah,Hashim Uda
Abstract
Purpose
The purpose of this study is to fabricate a transducer-based TiO2 interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for Escherichia. coli O157:H7 DNA detection.
Design/methodology/approach
Sol-gel was used to synthesize TiO2 nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.
Findings
TiO2 nanoparticles IDEs with 16-um gap size is able to detect DNA of E. coli in a dry state.
Originality/value
This paper describes IDEs for dry-state detection of E. coli O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.
Subject
Electrical and Electronic Engineering,Surfaces, Coatings and Films,Condensed Matter Physics,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials
Reference46 articles.
1. Agirtas, M.S., Altındal, A., Salih, B., Saydam, S. and Bekaroğlu, O. (2011), “Synthesis, characterization, and electrochemical and electrical properties of novel mono and ball-type metallophthalocyanines with four 9,9-bis(4-hydroxyphenyl)fluorine”, Dalton Transactions, Vol. 40 No. 13, pp. 3315-3324, available at: http://dx.doi.org/10.1039/C0DT01575J
2. Synthesis and characterization of titania nanoparticles via sol-gel method,2012
3. Titanium dioxide nanowires-based interdigitated electrodes for biomedical application,2012
4. Rapid and sensitive strategy for human papillomavirus (HPV) detection using a gene-based DNA nanobiosensor,2014
5. Fabrication of nanostructure-based copper oxide biosensor;Advanced Material Research,2015
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