Author:
Sharma Pranav,Mesci Pinar,Carromeu Cassiano,McClatchy Daniel R.,Schiapparelli Lucio,Yates John R.,Muotri Alysson R.,Cline Hollis T.
Abstract
Exosomes are thought to be released by all cells in the body and to be involved in intercellular communication. We tested whether neural exosomes can regulate the development of neural circuits. We show that exosome treatment increases proliferation in developing neural cultures and in vivo in dentate gyrus of P4 mouse brain. We compared the protein cargo and signaling bioactivity of exosomes released by hiPSC-derived neural cultures lacking MECP2, a model of the neurodevelopmental disorder Rett syndrome, with exosomes released by isogenic rescue control neural cultures. Quantitative proteomic analysis indicates that control exosomes contain multiple functional signaling networks known to be important for neuronal circuit development. Treating MECP2-knockdown human primary neural cultures with control exosomes rescues deficits in neuronal proliferation, differentiation, synaptogenesis, and synchronized firing, whereas exosomes from MECP2-deficient hiPSC neural cultures lack this capability. These data indicate that exosomes carry signaling information required to regulate neural circuit development.
Funder
HHS | NIH | National Institute of Mental Health
HHS | NIH | National Eye Institute
Publisher
Proceedings of the National Academy of Sciences
Cited by
177 articles.
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