Discovery of a rapidly evolving yeast defense factor, <i>KTD1</i> , against the secreted killer toxin K28-Reference-Cited by-同舟云学术

Discovery of a rapidly evolving yeast defense factor, KTD1 , against the secreted killer toxin K28

Published:2023-02-17 Issue:8 Volume:120 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Andreev Ilya¹^ORCID,Laidlaw Kamilla M. E.²³,Giovanetti Simone M.¹,Urtecho Guillaume⁴,Shriner Daniel⁵^ORCID,Bloom Joshua S.⁶⁷⁸⁹¹⁰,MacDonald Chris²³,Sadhu Meru J.¹^ORCID

Affiliation:

1. Computational and Statistical Genomics Branch, National Human Genome Research Institute, NIH, Bethesda, MD 20892

2. Biology Department, University of York, York YO10 5DD, UK

3. York Biomedical Research Institute, University of York, York YO10 5NG, UK

4. Molecular Biology Interdepartmental Doctoral Program, University of California, Los Angeles, CA 90095

5. Center for Research on Genomics and Global Health, National Human Genome Research Institute, NIH, Bethesda, MD 20892

6. Department of Human Genetics, University of California, Los Angeles, CA 90095

7. Department of Biological Chemistry, University of California, Los Angeles, CA 90095

8. HHMI, University of California, Los Angeles, CA 90095

9. Institute for Quantitative and Computational Biology, University of California, Los Angeles, CA 90095

10. Department of Computational Medicine, University of California, Los Angeles, CA 90095

Abstract

Secreted protein toxins are widely used weapons in conflicts between organisms. Elucidating how organisms genetically adapt to defend themselves against these toxins is fundamental to understanding the coevolutionary dynamics of competing organisms. Within yeast communities, “killer” toxins are secreted to kill nearby sensitive yeast, providing a fitness advantage in competitive growth environments. Natural yeast isolates vary in their sensitivity to these toxins, but to date, no polymorphic genetic factors contributing to defense have been identified. We investigated the variation in resistance to the killer toxin K28 across diverse natural isolates of the Saccharomyces cerevisiae population. Using large-scale linkage mapping, we discovered a novel defense factor, which we named KTD1 . We identified many KTD1 alleles, which provided different levels of K28 resistance. KTD1 is a member of the DUP240 gene family of unknown function, which is rapidly evolving in a region spanning its two encoded transmembrane helices. We found that this domain is critical to KTD1 ’s protective ability. Our findings implicate KTD1 as a key polymorphic factor in the defense against K28 toxin.

Funder

HHS | NIH | National Human Genome Research Institute

HHS | NIH | National Institute of General Medical Sciences

Howard Hughes Medical Institute

Wellcome Trust

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2217194120

Reference71 articles.

1. Toxic proteins in plants

2. J. Alouf, D. Ladant, M. R. Popoff, The Comprehensive Sourcebook of Bacterial Protein Toxins (Elsevier Inc., ed. 4, 2015), 10.1016/C2013-0-14258-4.

3. Animal protein toxins: origins and therapeutic applications

4. Evolutionary diversification of TTX-resistant sodium channels in a predator–prey interaction

5. Sodium channel mutation leading to saxitoxin resistance in clams increases risk of PSP

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