Contribution of cytoplasm viscoelastic properties to mitotic spindle positioning

Abstract

Significance The regulation of mitotic spindle positioning is a key process for tissue architecture, embryo development, and stem cells. To date, most models have assumed that spindles are positioned by forces exerted by polar cytoskeleton networks, like microtubule asters or actomyosin bundles. Here, using in situ magnetic tweezers to apply calibrated forces and torques to mitotic spindles in live dividing sea urchin cells, we found that the viscoelastic properties of the cytoplasm medium in which spindles are embedded can hold spindles in place and move them back if their original position is perturbed. These viscoelastic forces are large and may significantly participate in the force balance that position and orient mitotic spindles in many cell types.