Molecular mechanisms underlying metamorphosis in the most-ancestral winged insect

Abstract

Significance As caterpillars metamorphose to butterflies, insects change their appearance dramatically through metamorphosis. Some insects have an immobile pupal stage for morphological remodeling (homometaboly). Other insects, such as cockroaches, have no pupal stage, and the juveniles and adults are morphologically similar (hemimetaboly). Notably, among the most-ancestral hemimetabolous insects, dragonflies drastically alter their appearance from aquatic nymphs to aerial adults. In dragonflies, we showed that transcription factors Kr-h1 and E93 are essential for regulating metamorphosis as in other insects, while broad , the master gene for pupation in holometabolous insects, regulates a number of both nymph-specific genes and adult-specific genes, providing insight into what evolutionary trajectory the key transcription factor broad has experienced before ending up with governing pupation and holometaboly.