CSB-independent, XPC-dependent transcription-coupled repair in Drosophila

Abstract

Significance We have discovered that Drosophila , which does not have the canonical TCR homologs, does nevertheless carry out TCR as efficiently as organisms that do. Furthermore, using the XR-seq and in vivo excision assay we have also shown that both global repair and TCR in Drosophila are dependent on the XPC protein and in that regard, Drosophila excision repair is more similar to the monocellular eukaryotic yeast repair system than it is to multicellular eukaryotes. Finally, we have generated genome-wide single nucleotide repair maps of Drosophila for CPDs, (6-4) photoproducts, and cisplatin-d(GpG) adducts that should be a useful source for investigators working on DNA damage, repair, and mutagenesis in Drosophila .