The isolation of viable coccidial sporozoites

Author:

Jackson A. R. B.

Abstract

A method is described by which viable sporozoites of the sheep parasite,Eimeria arloingi, may be obtained in sterile solution and free of any extraneous vegetable matter. Faeces are washed through metal sieves and the oocysts separated by a gravity floatation technique. The oocysts are sterilized and floated free of any remaining faecal debris in a solution of sodium hypochlorite. Sporulation is induced by constant aeration in water. Excystation is effected by incubating firstly under an atmosphere of carbon dioxide and secondly in a solution of trypsin and sheep bile.I wish to thank Professor H. R. Carne for encouragement and advice, and Mr R. F. Jones for skilful technical assistance.

Publisher

Cambridge University Press (CUP)

Subject

Infectious Diseases,Animal Science and Zoology,Parasitology

Reference18 articles.

1. An improved method for sporulating oocysts in bovine faecal material;Hammond;Amer. J. vet. Res.,1944

2. A simple levitation method for the detection of hookworm ova;Willis;Med. J. Aust.,1921

3. Excystation of Eimeria Arloingi (Marotel, 1905): Stimuli from the Host Sheep

4. THE RECOVERY AND IDENTIFICATION OF THE FIRST STAGE LARVAE OF SHEEP NEMATODES

5. The suitability of various bacteria as food for hookworm larvae;McCoy;Amer. J. Hyg.,1929

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3