Author:
FRANÇA RAQUELI T.,DA SILVA ALEKSANDRO S.,WOLKMER PATRÍCIA,OLIVEIRA VITOR A.,PEREIRA MARIA E.,LEAL MARTA L. R.,SILVA CÁSSIA B.,NUNES MATHEUS A. G.,DRESSLER VALDERI L.,MAZZANTI CINTHIA M.,MONTEIRO SILVIA G.,LOPES SONIA T. A.
Abstract
SUMMARYThe aim of this study was to evaluate the activity of delta-aminolevulinate dehydratase (δ-ALA-D) in red blood cells of rats infected withTrypanosoma evansiand establish its association with haematocrit, serum levels of iron and zinc and lipid peroxidation. Thirty-six male rats (Wistar) were divided into 2 groups with 18 animals each. Group A was non-infected while Group B was intraperitoneally infected, receiving 7·5×106trypomastigotes per animal. Each group was divided into 3 subgroups of 6 rats and blood was collected during different periods post-infection (p.i.) as follows: day 5 (A1 and B1), day 15 (A2 and B2) and day 30 PI (A3 and B3). Blood samples were collected by cardiac puncture to estimate red blood cell parameters (RBC),δ-ALA-D activity and serum levels of iron, zinc and thiobarbituric acid reactive substances (TBARS). Rats in group B showed a significant (P<0·05) reduction of RBC count, haemoglobin concentration and haematocrit at days 5 and 15 p.i. The activity ofδ-ALA-D in blood was significantly (P<0·001) increased at days 15 and 30 p.i.δ-ALA-D activity in blood had a significant (P<0·05) negative correlation with haematocrit (r=−0·61) and haemoglobin (r=−0·70) at day 15 p.i. There was a significant (P<0·05) decrease in serum iron and zinc levels and an increase in TBARS levels (P<0·05) during infection. Theδ-ALA-D activity in blood was negatively correlated with the levels of iron (r=−0·68) and zinc (r=−0·57) on day 30 p.i. It was concluded that the increased activity ofδ-ALA-D in blood might have occurred in response to the anaemia in remission as heme synthesis was enhanced.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
8 articles.
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