Temperature-dependence of fish muscle AMP-aminohydrolase

Abstract

The effect of temperature on the rate of reaction catalysed by crude muscle AMP-aminohydrolase from two species of elasmobranch and two species of teleost fish has been investigated at low substrate concentration in the range of temperature 5–50 °C.The enzyme from teleost fish shows a very broad temperature optimum around 30 °C. The reaction rate of the enzyme from elasmobranch fish is strongly dependent on temperature. The optimum temperature for the enzyme from thornback ray lies around 40 °C. Both crude and 500-fold purified AMP-aminohydrolase from thornback ray show the same temperature-dependence.