Effect ofINSIG1on the milk fat synthesis of buffalo mammary epithelial cells

Abstract

AbstractWe hypothesized that insulin-induced gene 1 (INSIG1) affects milk fat synthesis in buffalo. For this reason, the protein abundance of INSIG1 in the mammary tissue of buffalo during the peak period of lactation and dry-off period was evaluated. The results showed that the expression of INSIG1 at the peak of lactation was lower than that in the dry-off period. To explore the role of INSIG1 in milk fat synthesis, the buffalo mammary epithelial cells (BMECs) were isolated and purified from buffalo mammary tissue, andINSIG1gene were overexpressed and knocked down by constructing the recombinant lentivirus vector ofINSIG1gene and transfecting into BMECs. Results revealed thatINSIG1overexpression decreased the expression ofINSIG2,SREBP,PPARG,SCD,GPAM,DGAT2andAGPAT6, which led to reduction of triglycerides (TAG) content in the cell. In contrast, knockdown ofINSIG1had a positive effect on mRNA expression of the above genes. Overall, the data provide strong support for a key role of INSIG1 in the regulation of milk fat synthesis in BMECs.