Genomic variability within laboratory and wild subspecies ofHeligmosomoides polygyrus

Abstract

AbstractGenomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematodeHeligmosomoides polygyruswere compared using RFLP and DNA/DNA hybridization techniques. Eight restriction endonuclease digests of the genomic DNA of the two subspecies were hybridized with heterologous ribosomal DNA probes and the total radio-isotope labelled DNA of the laboratory subspecies. DNA hybridization of the two subspecies ofH. polygyrusyielded different banding patterns when probed with the rDNA clones inPvuII digests and when total genomic DNA was used as the probe inHind III andPvuII digests. The remaining hybridization profiles of both subspecies were identical.