Effect of diet, sex and age on fatty acid metabolism in broiler chickens:n-3 andn-6 PUFA

Abstract

The PUFA metabolism in broiler chicken was studied through the whole body fatty acid balance method. Four dietary lipid sources (palm fat, Palm; soyabean oil, Soya; linseed oil, Lin; fish oil, Fish) were added at 3 % to a basal diet containing 5 % palm fat. Diets were fed to female and male birds from day 1 to either day 21 or day 42 of age. Birds fed the Lin diet showed a significantly higher 18 : 2n-6 accumulation compared with the other diets (85·2v.73·6 % of net intake), whereas diet did not affect 18 : 3n-3 accumulation (mean 63 % of net intake). Bioconversion of 18 : 2n-6 significantly decreased in the order Palm>Lin>Soya>Fish (4·7, 3·9, 3·4 and 1 % of net intake, respectively). The 18 : 3n-3 bioconversion on the Palm and Soya diets was similar and significantly higher than in broilers on the Lin diet (9·1v.5·8 % of net intake). The β-oxidation of 18 : 2n-6 was significantly lower on the Lin diet than on the other diets (10·8v.23·3 % of net intake), whereas β-oxidation of 18 : 3n-3 was significantly higher on the Fish diet than on the other diets (41·5v.27·3 % of net intake). Feeding fish oil suppressed apparent elongase and desaturase activity, whereas a higher dietary supply of 18 : 3n-3 and 18 : 2n-6 enhanced apparent elongation and desaturation activity on the PUFA involved in then-3 andn-6 pathway, respectively. Accumulation of 18 : 2n-6 and 18 : 3n-3 increased and β-oxidation decreased with age. Sex had a marginal effect on the PUFA metabolism.