Nutritional evaluation of kale (Brassica oleracea) diets:4. Responses to supplementation with synthetic S-methyl-L-cysteine sulphoxide (SMCO)

Abstract

SUMMARYSynthetic SMCO was added to basal diets of either fresh kale or lucerne at the concentrations 0, 0·2, 0·4, 0·8 and 1·6% D.M. and each diet offered ad libitum to groups of four lambs that were individually fed indoors. Within each group, two lambs were of haemoglobin genotype AA and two were AB. Initial live weight (W) was 21 kg and the experiment lasted for 6 weeks.SMCO supplementation depressed voluntary intake, body growth and wool growth, caused the development of haemolytic anaemia and produced kidney hypertrophy. Effects were much more severe when SMCO was added to a kale than to a lucerne diet, with the lowest SMCO intake of kale-fed lambs (0·3 g/kg W) producing marked detrimental effects whereas the highest SMCO intake of lucerne-fed lambs (0·7 g/kg W) produced only small and transient effects. The intake depressions were evident during the first week of SMCO supplementation and persisted throughout the full 6-week feeding period. Approximately 80 and 65% of the changes in body growth and wool growth could be accounted for by changes in intake, and it was concluded that the most important effect of SMCO in ruminant diets is as an intake depressant.It is suggested that the greater ratio of soluble to structural carbohydrate in kale (2·3) compared with lucerne (0·9) probably resulted in a microbial population developing in the rumen of kale-fed sheep that could metabolize SMCO to dimethyl disulphide at a faster rate than occurred in the rumen of lucerne-fed sheep. Dimethyl disulphide production is postulated as the cause of both the haemolytic anaemia and appetite depression. Plasma SMCO concentrations did not increase with increasing SMCO intake in sheep fed either diet, indicating that there must have been negligible absorption of this amino acid from tho digestive system.No haemoglobin C was produced by any of the lambs fed lucerne, and kale-fed lambs produced significant amounts only during weeks 5 and 6, with type AA lambs producing more than type AB lambs. However, there was no difference between AA and AB lambs in rate of decline in total haemoglobin concentration or upon voluntary intake, body growth and wool growth.