Abstract
AbstractBeing difficult to regenerate and maintain the seeds, the oleaginous bottle gourd was investigated using nine agromorphological traits and 31 amplified fragment length polymorphism (AFLP) markers. Specifically, the study was conducted to determine the intra-specific variability of a total of 173 accessions, which were identified from five agro-ecological regions from Côte d'Ivoire (Centre, East, North and South). Then, the genetic diversity and relationships within accessions were studied using AFLP markers. This characterization using both morphological and AFLP markers was realized in order to ultimately build a reliable core collection. The discriminant analysis, using nine quantitative traits, reveals plant length and seeds number per fruit as discriminating characteristics. From the accessions used for the agromorphological study, 148 were able to be differentiated by the AFLP markers. A range of 52 to 113 bands were amplified per primer combination. As revealed by the analysis of molecular variance (AMOVA), 28% of the total variation resides among accessions and 72% occurs within populations. The AMOVA computed in order to differentiate cultivars, displayed the same trends when no prior grouping of accessions was considered. The differentiation within cultivar (97%) was more than that, among cultivars (3%). Tree topologies inferred by neighbour-joining analysis reflected no clear cut off grouping.To group accessions, we used a Bayesian clustering analysis which exhibited two clusters. Using the informativeness of the primer combinations analysed in the present study, an orientation was given for the choice of the accessions which would be used to build a core collection.
Funder
Africa Biosciences Challenge Fund Fellowship
Publisher
Cambridge University Press (CUP)
Subject
Plant Science,Genetics,Agronomy and Crop Science
Reference86 articles.
1. EFFECTS OF FRUIT MATURITY ON OXIDATIVE STABILITY OFLAGENARIA SICERARIA(MOLINA) STANDL. SEED OIL EXTRACTED WITH HEXANE
2. Morphological and allozyme variation in a collection of Lagenaria siceraria (Molina) Standl. from Côte d'Ivoire;Koffi;Biotechnology, Agronomy, Society and Environment,2009
3. Molecular Diversity of a Germplasm Collection of Squash (
Cucurbita moschata
) Determined by SRAP and AFLP Markers
4. Increase in rice yield through the use of quality seeds in Bangladesh;Haque;African Journal of Agricultural Research,2012