The expression of propionicin PLG-1 gene (plg-1) by lactic starters

Abstract

Propionicin PLG-1 is a bacteriocin produced byPropionibacterium thoeniiP127. Such bacteriocin inhibits wide range of food-borne pathogens such as pathogenicEscherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Yersinia enterocoliticaand a strain ofCorynebacteriumsp. In the present study,plg-1gene expressing propionicin PLG-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. The PCR product containingplg-1gene was transferred to different lactic acid bacterial (LAB) strains using pLEB590 as a cloning vector to give the modified vector pLEBPLG-1. LAB transformants showed an antimicrobial activity againstEsch. coliDH5α (most affected strain),Listeria monocytogenes18116, andSalmonella enterica25566 as model pathogenic strains. Such LAB transformants can be used in dairy industry to control the food-borne pathogens that are largely distributed worldwide and to feed schoolchildren in the poor countries where dangerous epidemic diseases and diarrhoea prevail.