The effect of some variables on experimentalKlebsiellainfections in mice

Abstract

The virulence of nine strains ofKlebsiellawas tested in mice inoculated by peritoneal and oral routes, the latter being a convenient method of inducing pneumonic infections.At similar doses, shorter survival was observed in young mice. Injected by the intraperitoneal route, organisms from 16 hr. old cultures proved more virulent than those from older cultures, but no such difference was obtained orally. Repeated subculture of a virulent strain failed to attenuate its oral virulence and killed suspensions of the same strain were without effect. With only two exceptions, all strains were more virulent when administered orally than intraperitoneally. Wherever possible the concept ofrelativevirulence was employed in making statistical comparisons. It is, however, considered that virulence is an unsatisfactory taxonomic discriminant.We would like to thank Dr I. Friedmann, Dr F. Fulton and Dr G. Meynell for their helpful criticism. We are also indebted to Dr Lynne Reid for her comments and to Mr G. B. Newman for checking the calculations. Thanks are also due to Mr G. D. Breach and Mr G. Latchford for technical assistance, to Mr D. Connolly for preparation of graphs and Mrs A. Gorvin for secretarial assistance.