Location of genes for the utilization of acetamide, histidine and proline on the chromosome ofPseudomonas aeruginosa

Abstract

SUMMARYInterrupted mating methods were used to map the positions on thechromosomeof genes for the utilization of acetamide, histidine and proline. An entry time of 22 min was found for aputmarker determining the utilization of proline. Two groups ofhutmutants were studied. Mutationhut-109 was located at 18 min and mutationhutU108, which results in a defect in urocanase activity, mapped at 28 min.hutUwas shown by transduction with bacteriophage F116 to be linked toser-3 but not tomet-28 andilv-202, giving a map order ofhutU-ser-3-ilv-202-met-28.The amidase genes were assigned an entry time of 65–75 min. A method is described for examining late entry markers by interrupted mating.