Author:
Si Chen,Kun-Lun Huang,Wen-Tao Xu,Yuan Li,Yun-Bo Luo
Abstract
AbstractA rapid and accurate real-time quantitative polymerase chain reaction (real-time PCR) method with SYBR Green I was established for detectingEscherichia coliO157:H7. A pair of primers were designed to amplify theeaegene. The dissociation curves showed that the amplification product was very specific. The optimal conditions and standard curve were established. The result indicated that real-time PCR was 1000 times more sensitive than ordinary PCR.
Publisher
Cambridge University Press (CUP)
Subject
Agronomy and Crop Science,Biotechnology
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