Author:
DEPLANO STEFANIA,PEDEMONTE NICOLETTA
Abstract
This study presents a morphological and quantitative analysis
of displaced ganglion cells performed in the chick retina at
different stages of development (E11, E17, & P2). The
lipophilic dye DiI inserted into the optic nerve provided a
complete staining of displaced ganglion cell population and
of their dendritic trees. From 11 days of incubation (E11) all
neurons present a uniform morphology and are unmistakably
recognizable by the dimensions of their cell body and by the
presence of the axon. Their dendritic arborizations shape a
complex network through the retina. Cell bodies are randomly
distributed but dendritic trees of neighboring cells constantly
present two levels of dendritic overlap: about 50% of the cells
present a high degree of overlap, more than the 30% up to 95%
of their total area, while the other half overlaps less than
20%. The high degree of overlap identifies a cluster, which
is regularly formed by three and more rarely by four neurons
starting from 17 days of incubation (E17) onward and after birth.
From E17 onward, 94% of clusters is constantly formed by three
cells and 6% of the clusters by four neurons. The distribution
and the spatial arrangement of clusters are very regular, forming
a mosaic that gives rise to a precise retinal coverage. The
nearest-neighbor analysis unequivocally demonstrated that the
mosaic of clusters is the most regular array so far described.
This exact arrangement starts from 11 days of incubation and
is maintained and refined through the following stages of
development and after birth.
Publisher
Cambridge University Press (CUP)
Subject
Sensory Systems,Physiology
Cited by
2 articles.
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