Persistent environmental reservoirs on farms as risk factors forCampylobacterin commercial poultry

Abstract

SUMMARYCampylobacteris the most common known source of human bacterial enteritis in the developed world and poultry is considered the main source. Broilers often become colonized withCampylobacterduring rearing, and then contaminate the farm environment. The objective of this study was to identifyCampylobacter-positive environmental reservoirs on farms, as these pose a risk to broiler flocks becoming colonized withCampylobacter. We considered the temporal aspects of exposure and colonization. A longitudinal study monitored six conventional rearing farms over 2 years. The broiler flocks, catchers' equipment, vehicles, shed surrounds, shed entrance, other equipment, farm entrance, other animals, puddles, dead birds, mains water and drinkers were systematically sampled 2–4 times per flock. A multivariable generalized estimating equation model was used to assess associations between contaminated environmental sites and colonized broiler flocks. The associations were adjusted for confounders and other known risk factors. To further assess temporality of contamination, the sequence of contamination of the different environmental sites and the flocks was established. Contaminated shed entrances and anterooms, contaminated drinkers and shedding ofCampylobacterby other animals such as cattle, dogs, wildlife and rodents were significantly associated with positive flocks. The reservoir of ‘other animals’ was also the reservoir most commonly positive before the flock became colonized. The other sites usually became contaminated after the flock was colonized.