Synchronization ofE. coliO157 shedding in a grass-fed beef herd: a longitudinal study

Abstract

SUMMARYThis study aims to describe in detail the temporal dynamics ofE. coliO157 shedding and risk factors for shedding in a grass-fed beef herd. During a 9-month period, 23 beef cows were sampled twice a week (58 sampling points) andE. coliO157 was enumerated from faecal samples. Isolates were screened by PCR for presence ofrfbE,stx1andstx2. The prevalence per sampling day ranged from 0% to 57%. This study demonstrates that many members of the herd were concurrently sheddingE. coliO157. Occurrence of rainfall (P< 0·01), feeding silage (P< 0·01) and lactating (P< 0·01) were found to be predictors of shedding. Moving cattle to a new paddock had a negative effect on shedding. This approach, based on short-interval sampling, confirms the known variability of shedding within a herd and highlights that high shedding events are rare.