An electron microscope study of the body wall of the third-stage larva of Nippostrongylus brasiliensis

Abstract

The cuticle of the third-stage larva of Nippostrongylus brasiliensis consists of seven layers: an outer triple-layered membrane, a double-layered outer cortex, an inner cortex, a matrix layer, a striated layer and two fibril layers. In each ‘annule’ two fibres run transversely around the nematode and lie between the inner cortex and the matrix layer. There is no basement lamella.The hypodermis is a thin layer between the muscles and the cuticle, but expands to form the dorsal, ventral and lateral cords. The nerves lie between the plasma membrane of the hypodermis and the basement membrane or between the plasma membrane of the hypodermis and the sarcolemma of the muscles. The muscle cells are typical of those described previously for nematodes. The ‘myofibrils’ are apparently similar to those of Ascaris.An excretory canal is present in each lateral cord and is enclosed by the basement membrane but is not embedded in the hypodermal tissue. Numerous small vesicles appear to move across the wall of the excretory canal and open into the central lumen.I am grateful to Dr P. Tate, Dr R. W. Horne and Dr K. A. Wright for helpful discussions, to Professor C. P. Read and Dr A. Enders for the use of facilities at Rice University, Houston, Texas and to Professor J. D. Boyd for permission to use the electron microscope in the Department of Anatomy. Thanks are also due to Mr A. J. Page for technical assistance.