Sexual dimorphism of the cuticle and body-wall muscle in free-living mycophagous nematodes

Abstract

Sexual dimorphism in motility-related traits is widespread among animals, including several species of Nematoda. However, no study has examined motility-related structural components and compared them between sexes. We examined the motility-related components in four species: Bursaphelenchus conicaudatus Kanzaki, Tsuda and Futai, 2000; Bursaphelenchus rainulfi Braasch and Burgermeister, 2002; Bursaphelenchus doui Braasch, Gu, Burgermeister and Zhang, 2005; Parasitaphelenchus costati Kanzaki, Ekino, Ide, Masuya and Degawa, 2018. We measured the structure and amount of cuticle and body-wall muscles and estimated their relationship to body diameter or total cross-sectional area. Although no structural differences were observed in muscle, the relevant muscle area of B. doui and P. costati was significantly smaller in females than in males. This difference was greatest in P. costati. In all but B. doui, the relative cuticle thickness was significantly smaller in females than in males. Furthermore, only P. costati females had no striated basal zones in their cuticles; these are thought to be cross-linked proteins that provide strength to nematode cuticle during body movement. These results indicate that sexual dimorphism in motility-related structural components is present in P. costati and that females invest less energy in the components than do males.