Expression optimization of subunitfasGof 987P fimbriae in enterotoxigenicEscherichia coli(ETEC)

Abstract

AbstractFour bioengineered bacteria having thefasGgene were constructed using twoEscherichia coliexpression strains (BL21 (DE3) and Rossetta (DE3)) with different tRNA profiles and twoE. coliexpression vectors (pET28a(+) and pGEX-KG) to examine the influence of rare codons on the expression of thefasGgene. The induction expression result of bioengineered bacteria showed that the expression levels of target proteins in Rossetta (DE3), which contained the tRNAs of the rare codons, were higher than in BL21 (DE3), either using pET-fasGor pKG-fasG.