Comparison of the action of rumen bacteria on cell walls fromEragrostis tef

Abstract

SUMMARYA selection of the hemicellulose-utilizing rumen bacteria isolated by Henning (1979) were partially characterized. These and a number of other cellulolytic organisms were incubatedin vitrowith teff (Eragrostis tef) cell walls, and the extent of solubilization and utilization by the bacteria of individual cell wall sugars was determined. Results were compared with the action of the bacteria on isolated xylan and cellulose.The cellulolytic rumen bacteria solubilized more of both cellulosic and hemicellulosic sugars in the cell wall than the non-cellulolytic organisms. Bacteria which were unable to solubilize isolated cellulose could also degrade very little of the cell wall cellulose, and this appeared to limit the amount of cell wall hemicellulose which could be attacked.There was no direct relationship between the extent of degradation of isolated xylan and solubilization of cell wall hemicellulose, but those xylanolytic organisms which produced freely diffusible enzymes (as evidenced by production of clearings in 3 % xylan-agar) were more effective in attacking the cell wall than those which did not.Examination of thin sections in the electron microscope showed no relationship between attachment of bacteria to the cell walls and ability to degrade them.