Energy Conservation and the Promotion ofLegionella pneumophilaGrowth: The Probable Role of Heat Exchangers in a Nosocomial Outbreak

Author:

Bédard Emilie,Lévesque Simon,Martin Philippe,Pinsonneault Linda,Paranjape Kiran,Lalancette Cindy,Dolcé Charles-Éric,Villion Manuela,Valiquette Louis,Faucher Sébastien P.,Prévost Michèle

Abstract

OBJECTIVETo determine the source of aLegionella pneumophilaserogroup 5 nosocomial outbreak and the role of the heat exchanger installed on the hot water system within the previous year.SETTINGA 400-bed tertiary care university hospital in Sherbrooke, Canada.METHODSHot water samples were collected and cultured forL. pneumophilafrom 25 taps (baths and sinks) within wing A and 9 taps in wing B. Biofilm (5) and 2 L water samples (3) were collected within the heat exchangers forL. pneumophilaculture and detection of protists. Sequence-based typing was performed on strain DNA extracts and pulsed-field gel electrophoresis patterns were analyzed.RESULTSFollowing 2 cases of hospital-acquired legionellosis, the hot water system investigation revealed a large proportion ofL. pneumophilaserogroup 5 positive taps (22/25 in wing A and 5/9 in wing B). High positivity was also detected in the heat exchanger of wing A in water samples (3/3) and swabs from the heat exchanger (4/5). The outbreak genotyping investigation identified the hot water system as the source of infections. Genotyping results revealed that all isolated environmental strains harbored the same related pulsed-field gel electrophoresis pattern and sequence-based type.CONCLUSIONSTwo cases of hospital-acquired legionellosis occurred in the year following the installation of a heat exchanger to preheat hospital hot water. No cases were reported previously, although the sameL. pneumophilastrain was isolated from the hot water system in 1995. The heat exchanger promotedL. pneumophilagrowth and may have contributed to confirmed clinical cases.Infect. Control Hosp. Epidemiol.2016;1475–1480

Publisher

Cambridge University Press (CUP)

Subject

Infectious Diseases,Microbiology (medical),Epidemiology

Reference26 articles.

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