Use of Flow Cytometry To Monitor Legionella Viability-Reference-Cited by-同舟云学术

Use of Flow Cytometry To Monitor Legionella Viability

Published:2008-12-15 Issue:24 Volume:74 Page:7813-7816
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Allegra Séverine¹,Berger Françoise¹,Berthelot Philippe¹,Grattard Florence¹,Pozzetto Bruno¹,Riffard Serge¹

Affiliation:

1. Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), EA3064, Faculté de Médecine J. Lisfranc, Université Jean Monnet, Saint-Etienne, France

Abstract

ABSTRACT Legionella viability was monitored during heat shock treatment at 70°C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01364-08

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