Abstract
Ceratitis capitata (medfly) is one of the most devastating crop pests worldwide. The Sterile Insect Technique (SIT) is a control method that is based on the mass rearing of males, their sterilization, and release in the field. However, the effectiveness of the technique depends on the quality of the released males and their fitness. We previously isolated and selected a probiotic bacteria (Enterobacter sp.), from wild-caught medflies, according to criteria that improved biological quality traits of reared medfly males.We firstly evaluated the impact of the irradiation on the expression of different immune and stress genes in the medfly sterile males. Expression was measured at differents time points ranging from 0 to 168 h after irradiation to capture the response of genes with distinct temporal expression patterns. Then, we supplemented the larval diet with previously isolated Enterobacter sp.strain, live and autoclaved at various concentrations to see whether the probiotic treatments affect, through their protective role, the gene expression level, and quality traits. The irradiation had significant effect on the genes attacin, cecropin, PGPR-LC, hsp23, and hsp70 level expression. The expression of attacin and PGPR-LC was up-regulated while that of cecropin was down-regulated. Hsp genes showed decreased levels between 0 and 18 h to peak at 72 h. However, the supplementation of the probiotic strain, either live or autoclaved, was statistically significant only for attacingene. However, significant interaction time x probiotic was noticed for attacin, cecropin, hsp23 and hsp70. The probiotic treatments also improved the quality control parameters like pupal weight. From this work we can conclude that a consortium of parabiotics (autoclaved probiotics) treatment will be recommended in insectaries considering both the beneficial effects on mass reared insects and its general safety for insectary workers and for environment.
Funder
Ministère de l’Enseignement Supérieur et de la Recherche Scientifique
Ministère de l'Education Nationale, de la Formation professionnelle, de l'Enseignement Supérieur et de la Recherche Scientifique
Publisher
Public Library of Science (PLoS)
Reference104 articles.
1. Host plants of Mediterranean fruit fly (Diptera: Tephritidae) on the island of Hawaii (1949–1985);N.J. Liquido;J Econ. Entomol,1990
2. Liquido, N.J., McQuate, G.T., Suiter, Medhost, K.A. An encyclopedic bibliography of the host plants of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), Version 1.1. United States Department of Agriculture, Center for Plant Health Science and Technology, Raleigh, N.C: 2013.
3. Thomas, M.C., Heppner, J.B., Wooduff, R.E., Weems, H.V., Steck, G.J. and Fasulo, T.R. Mediterranean fruit fly, Ceratitis capitata (Weidemann) (Insecta: diptera, tephritidae). University of Florida, IFAS Ext. EENY. 2013, p.214.
4. Field efficacy of Ceratitis capitata (Diptera: Tephritidae) mass trapping technique on clementine groves in Spain;M.T. Martinez-Ferrer;J. Appl Entomol,2012
5. Acute pesticide poisoning: a proposed classification tool;J. Thundiyil;Bull World Health Organ,2008
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