Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches

Author:

Green Stefan J.,Venkatramanan Raghavee,Naqib Ankur

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference35 articles.

1. 16S/23S rRNA sequencing;D Lane;Nucleic acid techniques in bacterial systematics,1991

2. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms;JG Caporaso;The ISME journal,2012

3. Miniprimer PCR, a new lens for viewing the microbial world;TA Isenbarger;Applied and Environmental Microbiology,2008

4. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA;G Muyzer;Applied and Environmental Microbiology,1993

5. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA;JR Marchesi;Applied and Environmental Microbiology,1998

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